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Image Search Results
Journal: Kidney Diseases
Article Title: Treatment of Membranous Nephropathy by Disulfiram through Inhibition of Podocyte Pyroptosis
doi: 10.1159/000524164
Figure Lengend Snippet: DSF alleviated C3a/C5a-induced podocyte injury by inhibiting pyroptosis. Podocyte injury was induced with C3a (50 nM) and C5a (50 nM). The inhibitory effects of DSF (250 nM) on C3a/C5a-induced pyroptosis were examined. a Representative images of GSDMD(N)/ZO-1/Nucleus (DAPI) triple immunofluorescent staining of treated podocytes. Scale bars = 20 μm. b Representative Western Blot images of GSDMD, NF-κB p65, p-NF-κB p65 (Ser536), NLRP3, ASC, Caspase-1, IL-18 and the internal control (GAPDH) in treated podocytes. c IL-18 release in treated podocytes was detected. d, e Representative images of PI/Nucleus (DAPI) double fluorescent staining of treated podocytes ( d ) and percentage of PI-positive cells ( e ); arrows, PI-positive cells; scale bars = 40 μm. f LDH release in treated podocytes was detected. The data above represent three independent experiments in duplicate and are shown as the mean ± SD, and ANOVA with LSD-t test (equal variances assumed) or Welch's test with Dunnett's T3 test (equal variances not assumed) was used for multiple comparisons among groups. *, p < 0.05; **, p < 0.01.
Article Snippet: IL-18 release in the culture supernatant of the podocytes and the serum IL-1β/IL-18 levels of rats were tested using human IL-18 ELISA and
Techniques: Staining, Western Blot
Journal: Kidney Diseases
Article Title: Treatment of Membranous Nephropathy by Disulfiram through Inhibition of Podocyte Pyroptosis
doi: 10.1159/000524164
Figure Lengend Snippet: DSF inhibited the renal pyroptosis signaling pathway in PHN rats. a Representative renal GSDMD(N)/Synaptopodin and GSDMD(N)/ZO-1 double immunofluorescent staining of the rats ( n = 6) in each group; Scale bars = 20 μm. b, c Representative renal immunohistochemical staining ( b ) and semiquantification based on the glomerular IOD/area of GSDMD(N), NF-κB p65, p-NF-κB p65 (Ser536), NLRP3, ASC, Caspase-1, IL-1β, and IL-18 ( c ) of the rats ( n = 6) in each group; Scale bars = 20 μm. d Relative mRNA levels of glomerular GSDMD, NLRP3, ASC, Caspase-1, IL-1β and IL-18 of the rats ( n = 6) in each group. e Representative Western Blot images of renal GSDMD, NF-κB p65, p-NF-κB p65 (Ser536), nuclear NF-κB p65, nuclear p-NF-κB p65 (Ser536), NLRP3, ASC, Caspase-1, Caspase-1 p20, IL-1β, IL-1β (mature form), IL-18 and the internal control (GAPDH, Histone H3) of the rats ( n = 6) in each group. Serum IL-1β ( f ) and IL-18 ( g ) of the rats ( n = 6) in each group on days 1, 5, 8, 15 after model establishment. The data above are shown as the mean ± SD ( c, d, f, g ) and were compared to the PHN group ( f, g ). ANOVA with LSD-t test (equal variances assumed) or Welch's test with Dunnett's T3 test (equal variances not assumed) was used for multiple comparisons among groups. *, p < 0.05; **, p < 0.01.
Article Snippet: IL-18 release in the culture supernatant of the podocytes and the serum IL-1β/IL-18 levels of rats were tested using human IL-18 ELISA and
Techniques: Staining, Immunohistochemical staining, Western Blot
Journal: Frontiers in tropical diseases
Article Title: Adjuvants Differentially Modulate the Immunogenicity of Lassa Virus Glycoprotein Subunits in Mice
doi: 10.3389/fitd.2022.847598
Figure Lengend Snippet: Cytokine secretion measured from mouse splenocytes using a FluoroSpot assay. Geometric mean (GMT) with 95% confidence intervals of (A) IL-2, (B) TNF-α, (C) IFN-γ and (D) polyfunctional cytokine secretion from splenocytes harvested from mouse groups one-week post-dose 2 and stimulated with a LASV GP peptide pool for 24 hours. (n=3 for the ISA 720 groups, n=1-2 for LiteVax groups, and n=3-4 for GPI-0100 groups) A two-way ANOVA, followed by a Tukey’s multiple comparison was used to compare the number of cytokine-secreting cells from immunized groups to the group receiving LASV GP alone, and between different adjuvant formulations of the same antigen concentration, respectively. (*p < 0.05, **p < 0.01).
Article Snippet: The FluoroSpot assay was performed using mouse IFN-γ/TNF-α/IL-2
Techniques: Flurospot, Comparison, Adjuvant, Concentration Assay